Biology Test 194

Question 1
4 / -1

Stirred-tank bioreactors have been designed for :

A
availability of oxygen throughout the process

B
ensuring anaerobic conditions in the culture vessel

C
purification of product

D
addition of preservatives to the product

Question 2
4 / -1

What is the criteria for DNA fragments movement on agarose gel during gel electrophoresis ?

A
The smaller the fragment size, the farther it moves

B
Positively charged fragments move to farther end

C
Negatively charged fragments do not move

D
The larger the fragment size, the farther it moves

Question 3
4 / -1

Given below diagram showing cloning vector pBR-322. Mark the incorrect statement :-

A
Label 'a' represent ROP site, which codes for the proteins involved in the replication of plasmid

B
Bam H 1 site is located in amp^R region

C
Label 'b' repersent 'ori' site

D
Sal I site is located in tet^R region

Question 4
4 / -1

Which of the following is a wrong match?

A
Taq polymerase – Thermostable enzyme

B
Gene gun – Gold or tungston microparticles

C
Agrobacterium tumefaciens – T-DNA

D
EcoRI – Exonuclease

Question 5
4 / -1

Restriction site of EcoRI is GAATTC. This enzyme cuts between :-

A
G and A

B
T and C

C
G and C

D
T and T

Question 6
4 / -1

After the formation of product in the bioreactiors it undergoes through some processes, before a finished product to be ready for marketing is called :-

A
Elution

B
Upstream processing

C
Downstream processing

D
Transformation

Question 7
4 / -1

The method in which alien DNA is directly injected into the nucleus of an animal cell is known as :-

A
Biolistic

B
Micro-injection

C
Electroporation

D
Heat-shock treatment

Question 8
4 / -1

Which type of bioreactor is usually cylindrical or with a curved base to facilitate the mixing of the contents ?

A
Sparged stirred tank bioreactor

B
Simple stirred tank bioreactor

C
Both (1) & (2)

D
None of these

Question 9
4 / -1

Read the following statements and select the incorrect ones.

(i) 'Bt' in 'Bt cotton' indicates that it is a genetically modified crop produced by biotechnologically

(ii) Golden rice is a transgenic variety which is rich in vitamin A.

(iii) 'Flavr Savr' transgenic tomatoes remain fresh for a longer period than a normal tomato variety.

(iv) Anticoagulant 'hirudin' is being produces from transgenic Brassica napus seeds.

A
only (i)

B
(ii) and (iii)

C
(iii) and (iv)

D
(iv) and (i)

Question 10
4 / -1

Nematode resistant plant is formed by :-

A
Micropropagation

B
Gene silencing using RNAi

C
Transformation by retrovirus

D
Biolistics

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Biology Test 19...

Question 1

availability of oxygen throughout the process

ensuring anaerobic conditions in the culture vessel

purification of product

addition of preservatives to the product

Question 2

The smaller the fragment size, the farther it moves

Positively charged fragments move to farther end

Negatively charged fragments do not move

The larger the fragment size, the farther it moves

Question 3

Label 'a' represent ROP site, which codes for the proteins involved in the replication of plasmid

Bam H 1 site is located in ampR region

Label 'b' repersent 'ori' site

Sal I site is located in tetR region

Question 4

Taq polymerase – Thermostable enzyme

Gene gun – Gold or tungston microparticles

Agrobacterium tumefaciens – T-DNA

EcoRI – Exonuclease

Question 5

G and A

T and C

G and C

T and T

Question 6

Elution

Upstream processing

Downstream processing

Transformation

Question 7

Biolistic

Micro-injection

Electroporation

Heat-shock treatment

Question 8

Sparged stirred tank bioreactor

Simple stirred tank bioreactor

Both (1) & (2)

None of these

Question 9

only (i)

(ii) and (iii)

(iii) and (iv)

(iv) and (i)

Question 10

Micropropagation

Gene silencing using RNAi

Transformation by retrovirus

Biolistics

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Question Analysis

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Bam H 1 site is located in amp^R region

Sal I site is located in tet^R region